Composite

Part:BBa_K3196031:Design

Designed by: Hao Qiu   Group: iGEM19_HUST-China   (2019-10-16)


pGAP-Kozak-FLO10 pro-pelA-His tag-TT-Pgpda-Kozak-FLO10-SLAC-His tag-TT-Pgas-Alkali-TT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1527
    Illegal BamHI site found at 478
    Illegal BamHI site found at 2346
    Illegal BamHI site found at 2357
    Illegal BamHI site found at 2368
    Illegal BamHI site found at 3720
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2807
    Illegal SapI site found at 238


Design Notes

d


Source

d

References